Uji Aktivitas Antibakteri Ekstrak Daun Pepaya Jepang (Cnidoscolus aconitifolius) Dengan Metode Difusi Agar
DOI:
https://doi.org/10.59680/ventilator.v1i4.839Keywords:
Japanese papaya leaves, Antibacterial, Staphylococcus aureusAbstract
Japanese papaya leaves (Cnidoscolus aconitifolius) contain active compounds such as flavonoids, tannins, saponins, alkaloids and terpenoids which have the potential to have antibacterial activity. The aim of this research is to determine the antibacterial activity of Japanese papaya leaves against Staphylococcus aureus bacteria. Extraction of Japanese papaya leaves has been carried out using the maceration method and 96% ethanol solvent. The antibacterial activity test used the agar diffusion method using a cylindrical plate. Sterile distilled water was used as a negative control while as a positive control the antibiotic ciprofloxacin was used. The concentrations of Japanese papaya leaf ethanol extract used in testing were 10%, 20%, and 30%. The research results showed that Japanese papaya leaf ethanol extract with a concentration of 30% had the widest inhibition zone diameter, namely 17.296 mm. Meanwhile, at a concentration of 20%, the average inhibitory zone diameter was 15,222 mm, and at a concentration of 10%, the average inhibitory zone diameter was 13,018 mm. These three concentrations were included in the strong category based on Greenwood classification.
References
Afiffurahman, Samadin, K. H., & Aziz, S. (2014). Pola Kepekaan Bakteri Staphylococcus aureus terhadap Antibiotik Vancomycin di RSUP Dr. Mohammad Hoesin Palembang. Majalah Kedokteran Sriwijaya, 46(4), 266-277.
Aziz, T., Johan, M. E. G., & Sri, D. (2018). Pengaruh Jenis Pelarut, Temperatur dan Waktu Terhadap Karakterisasi Pektin Hasil Ekstraksi dari Kulit Buah Naga (Hylocereuspolyrhizus). Jurnal Teknik Kimia, 24(1), 17-27.
Jinghua, M., Gaizhuang, L., & Qiaoli, C. (2017). Pathogens and Antibiotic Resistance of Children With Community-acquired Pneumonia. Biomedical Research, 28(20), 8839-8843.
Inayatullah, S. (2012). Efek Ekstrak Daun Sirih Hijau (Piper betle L.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Skripsi.
Indang, N., Guli, M. M., & Alwi, M. (2013). Uji Resistensi dan Sensitivitas Bakteri Salmonella thypi pada Orang yang Sudah Pernah Menderita Demam Tifoid Terhadap Antibiotik. Biocelebes, 7(1).
Izza, A. R. F., Tambunan, F. M. A., & Maulina, D. (2023). Karakterisasi Dan Skrining Fitokimia Buah Lada Putih (Piperis albi fructus). Indonesian Journal of Health Science, 3(1), 1-6.
Purwanto, S. (2015). Uji Aktivitas Antibakteri Fraksi Aktif Ekstrak Daun Senggani (Melastoma malabathricum L) Terhadap Escherichia coli. Jurnal keperawatan Sriwijaya, 2(2), 84-92.
Ramdhani, F. (2023). Aktivitas Antibakteri Ekstrak Daun dan Batang Tanaman Pepaya Jepang (Cnidoscolus aconitifolius (Mill.) IM Johnst Terhadap Bakteri Staphylococus aureus ATCC 29213. Doctoral dissertation.
Salamah, N., & Widyasari, E. (2015). Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) dengan Metode Penangkapan Radikal 2, 2’-difenil-1-pikrilhidrazil. Pharmaciana, 5(1), 25-34.
Salim, H. H. U., & Soleha, T. U. (2018). Pengaruh Aktivitas Antimikroba Ekstrak Bawang Putih (Allium sativum) Terhadap Bakteri Gram Positif (Staphylococcus aureus) dan Gram Negatif (Escherichia coli) Secara In Vitro. Jurnal Medula, 7(5), 66-70.
Sengupta, S., & Chattopadhyay, M. K. (2012). Antibiotic Resistance of Bacteria: A Global Challenge. Resonance, 17, 177-191.
Setiawati, A. (2015). Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus Terhadap Amoxicillin Menggunakan Metode Adaptif Gradual. Jurnal Farmasi Indonesia, 7(3).
Silalahi, M. (2021). Bioactivity and Uses of Cnidoscolus aconitifolius (Mill.) IM Johnst. World Journal of Biology Pharmacy and Health Sciences, 7(3), 057-064.
Sulvita, N. (2018). Efektivitas Minyak Jabbatussauda (Nigella sativa) terhadap Pertumbuhan Staphylococcus aureus. UMI Medical Journal, 3(2), 14-24.
Sumampouw, O. J. (2018). Uji Sensitivitas Antibiotik terhadap Bakteri Escherichia coli Penyebab Diare Balita di Kota Manado. Journal of Current Pharmaceutical Sciences, 2(1), 104-110.
Toy, T. S., Lampus, B. S., & Hutagalung, B. S. (2015). Uji Daya Hambat Ekstrak Rumput Laut Gracilaria sp Terhadap Pertumbuhan Bakteri Staphylococcus aureus. e-GiGi, 3(1).
Trisia, A., Philyria, R., & Toemon, A. N. (2018). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kalanduyung (Guazuma ulmifolia Lam.) terhadap Pertumbuhan Staphylococcus aureus dengan Metode Difusi Cakram (Kirby-Bauer). Anterior Jurnal, 17(2), 136-143.
Wikansari, N. (2012). Pemeriksaan Total Kuman Udara dan Staphylococcus aureus di ruang Rawat Inap Rumah Sakit X Kota Semarang. Jurnal Kesehatan Masyarakat Universitas Diponegoro, 1(2), 18795.
