Uji Aktivitas Antioksidan Ekstrak Kulit Buah Mangga Arumanis (Mangifera Indica L.) dengan Metode DPPH Serta Uji Toksisitasnya
DOI:
https://doi.org/10.59680/ventilator.v2i3.1438Keywords:
Mangifera indica L, DPPH, Antioxidant, ToxicityAbstract
Arumanis mango rind (Mangifera indica L) usually becomes organic waste, but contains flavonoid secondary metabolites. Arumanis mango rind extract was chosen so that it can be used as an antioxidant compound, which is an antidote to free radicals. The aim of this research was to examine the antioxidant activity and toxicity tests of arumanis mango rind extract. The result is that the maximum absorption wave of DPPH is 518 nm, with an absorbance of 0.788. The results of comparative antioxidant activity for gallic acid obtained IC50 = 4.424 µg/mL, while the arumanis mango rind extract sample had an IC50 value = 18.294 µg/mL. For toxicity test results, the LC50 value = 169.043 µg/mL. Based on the research results obtained, it can be concluded that the antioxidant activity of arumanis mango rind extract is classified as very strong (<50 µg/mL) and the toxicity is classified as toxic (<1,000 µg/mL).
References
Abdullah, Al-Shwyeh H, AS Mohmamad, R Abdullah, MES. Mirghani and M Al- Qubaisi. 2014. Cytotoxoc Effects of Mangifera Indica L. Kernel Extract On Human Breast Cancer (MCF-7) And MDA-MB-231 Cell Lines) and Bioactive Constituentsi in the Crude Extract. BMC Complementary and Alternative Medicine. 14:199. 8-9
Aderiyanti Risma. 2022. Studi Perbandingan Metode Pengukuran Antioksidan. Skripsi Fakultas Tarbiyah Dan Keguruan Pendidikan Biologi. Lampung. Universitas Islam Negeri Raden Intan Lampung. 6-10
Alam, G. 2002. Brine Shrimp Lethality Test (BSLT) Sebagai Bioassay Dalam Isolasi Senyawa Bioaktif dari Bahan Alam. Majalah Farmasi dan Farmakologi. 6(2):432-435
Alkizim, Faraj Omar, D Matheka, FK Abdulrahman and A Murithi. 2012. Inhibitory Effect of Mangifera indica on Gastrointestinal Motility. Medicinal Chemistry and Drung Discovery. 2(1): 9-16.
Annis, A., Novianty, Y., & Hepiyansori, H. (2020). Variasi Kosentrasi Ekstrak Etanol Kulit Buah Mangga Harum Manis (Mangifera Indica L Var. Arum Manis) Terhadap Formula Sediaan Gel Hand Sanitizer Sebagai Antibakteri. Doctoral Dissertation, Stikes Al-Fatah Bengkulu. 71-72 Kimia dan Pendidikan Kimia, 1(3), 157-163.
Dachriyanus. 2004. Analisis Struktur Senyawa Organik Secara Spektroskopi. Cetakan Pertama. Padang: Universitas Andalas. 39-40
Daun Mangga Arumanis (Mangifera Indica L.) Pada Mencit Swiss Webster Jantan Dengan Metode Tes Toleransi Glukosa Oral (Ttgo). Jurnal Scientifica Unsiba, 2, 297-303.
Departemen Kesehatan RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Direktorat Pengawasan Obat Tradisional. 10-11
FAOSTAT. 2018. Food and agriculture organization of the United Nations. http://www.fao.org/faostat/en/#ranki ngs/countries_by_commodity. [8 Oktober 2018]. 58
Fitranto R., Nanang Dwi Wahyono dan Yossi Wibisono. 2020. Strategi Pengembangan Pemasaran Buah Mangga Arumanis 143 PT. Trigatra Rajasa Situbundo Jawa Barat. 58Jurnal Agribisnis Indonesia (Journal of Indonesian Agribusiness), 8(1), 58-68
Harborne, J.B. 1987. Metode Fitokimia Penentuan Cara Modern Menganalisis Tumbuhan, Cetakan Ke-2, Diterjemahkan oleh K.Padmawinata dan I.Soediro, Bandung: ITB. 166-169
Ifmaily, I., Firla, A., & Fitriani, P. R. (2023). The Effect of Arumanis Mango Rind (Mangifera indica L) Extract as Antidiabetic in Rats Model. JSFK (Jurnal Sains Farmasi & Klinis), 256-263.
Joshua, Mushore dan Matuvhunye Takudzwa. 2013. Antibacterial Properties of Mangifera indica on Staphylococcus Aureus. African Journal of Clinical and Experimental Biology. 14(2): 62-74.
Jun, M., Fu, H.-Y., Hong, J., Wan, X., Yang, C. S., & Ho, C.-T. (2003). Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria lobata Ohwi). Journal of Food Science, 68(6), 2117–2122. https://doi.org/10.1111/j.1365- 2621.2003.tb07029.x
Liochev, S.I., 2013, Reactive Oxygen Species and The Free Radical Theory Of Aging, Free Radical Biology and Medicine,60, 1-40.
Maimunah, Siti. 2021. Skripsi Pengaruh Waktu Maserasi Terhadap Kadar Alkaloid Total Pada Brokoli (Brassica Oleracea Var. Italica) Dengan Metode Spektrofotometri UV-Vis. Yogyakarta, Sekolah Tinggi Ilmu Kesehatan Akbidyo. 12-13.
Masibo, M & He, Q. 2008, Major Mango Polyphenols and Their Potential Significance to Human Health, Comprehensive Reviews In Food Service and Food Safety, 7. 309-19.
McLaughlin. 1982. “Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents”, Planta Medica. 45:31-34.
Meyer, U.N., N.R. Ferigni, J.E. Putnam, L.B. Ja Cobsen, D.E. Nichols, and J.L.
Molyneux P. 2004. The Use of the Stable Free Radical 1,1 Diphenyl. 2 Picrilhydrazil (DPPH) For Estimating Antioxidant Activity. Journal Science and Technology: 26:211-219.
Mulangsri DAK., Budiarti A, dan Saputri EN. 2017. Aktivitas Antioksidan Fraksi Dietileter Buah Mangga Arumanis (Mangifera indica L.) dengan Metode DPPH. Jurnal Pharmascience. 4(1) : 85-93.
Noer, S., Pratiwi, R. D., Gresinta, E., Biologi, P., & Teknik, F. (2018). Penetapan Kadar Senyawa Fitokimia (Tanin, Saponin dan Flavonoid) Sebagai Kuersetin Pada Ekstrak Daun Inggu (Ruta angustifolia L.). Jurnal Eksakta, 18(1), 19-29
Noviyanty, Y. (2020). Profil Fitokimia dari Ekstrak Etanol Kulit Buah Mangga Arum Manis (Mangifera Indica L.). Jurnal Ilmiah Pharmacy, 7(2), 242- 254.
Pourmorad, F., Hosseinimerh, S. J, & Shshabimajd, N. 2006. Antioxidant Activity, Phenol And Flavonoid Contect Of Some Selected Iranian Medicinal Plants, African Journal Of Biotechnology, 5 (11): 1142-1145.
Pratiwi Y, Sri Sumarsih, Windah Febria Windi. 2012. Uji Toksisitas Limbah Cair Laundry Sebelum dan Sesudah Diolah Dengan Tawas Dan Karbon Aktif Terhadap Bioindicator (Cyprinuscarpio L). Prosiding Seminar Nasional Aplikasi Sains & Teknologi (SNAST) Periode III. 2998-299
Rajan, S, H Suganya, T Thirunalasundari and S Jeeva. 2012. Antidiarrhoeal Efficacy of Mangifera Indica Seed Kernel on Swiss Albino Mice Asian Pacific. Journal of Tropical Medicine. 5(8): 630-633.
Saputra, A., Gani, A., & Erlidawati. (2017). Uji Aktivitas Antioksidan Daun Gulma Siam (Chromoleana Odorata L.) Dengan Metode 1,1-Diphenyl-2- Picrylhidrazyl. Jurnal Ipa Dan Pembelajaran Ipa (Jipi), 1(2), 131-142.
Senja RY, Issusolaningtyas E, Nugroho AK, Setyowati EP. 2014. the Comparisor. of Extraction Methoda and Solvent Variation on Yield and Antioxidant Activity of Brassia Oleracea L. Var. Capitata F. Rubra Extract. Traditional Medicine Journal. 19.(1): 43-48.
Shah, KA, MB Patel, RJ Patel and PK. Parmar. 2010. Mangifera indica (mango). Pharmacognosy Review. 4(7): 42-48..
Syah, I. S., Suwendar, & Mulqie, L. 2015. Uji Aktivitas Antidiabetes Ekstrak Etanol
Tian Yang., Wang., Qing Li., Kai-Shun Bi. 2018. Bioactive Flavonoids in Medicinal Plants: Structure, Activity and Biological Fateasian. Journal of Pharmaceutical Sciences, 13, 12-23.
Werdasari, Asri. 2014. Peran Antioksidan Bagi Kesehatan. Jurnal Biotek Medisiana Indonesa. 3(2), 59-69.
Widowati, Retno. 2008. Keberadaan Bakteri Vibrio parahaemolyticus pada Udang yang Dijual di Rumah Makan Kawasan Pantai Pangandaran. Vis Vitalis. 01, http://download.portalgaruda.org/article.php?article=59771&val=4487. Diakses pada 1 Maret 2017. 26-27
Wulandari dan I. Sulistyarini, 2018. Antibacterial Activity Test Of Extract Ethanol Mango Arum Manis Skin (Mangifera indica L.) On Methicillin Resistant Staphylococcus aureus (MRSA). Jurnal Media Farmasi Indonesia. 13 (2).
Yakubu, MT and SS Salimon. 2015. Antidiarhoeal Activity of Aqueous Extract of Mangifera indica L. Leaves in Female Albino Rats. Journal of Ethnopharmacology. 163: 135-141.
